A reversible fluorescence chemosensor for sequentially quantitative monitoring copper and sulfide in living cells.

We report a novel, selective and sensitive strategy for the sequentially "ON-OFF-ON" fluorescent detection of Cu(2+) and S(2-) based on a fluorescein derivative, FL. The specific binding of FL towards Cu(2+) in aqueous and biological media led to the intensive green fluorescence quenching and a notable increase of the absorbance maximum at 480 nm. In the presence of S(2-), the intensity and overall pattern of the fluorescence emission and UV-vis spectra of FL-Cu(2+) ensemble were recovered since the abolishment of paramagnetic Cu(2+). This displacement approach exhibited highly specificity, and sensitivity with detection limits of 3 nM for Cu(2+) and 150 nM for S(2-). The fluorescence "ON-OFF-ON" circle can be repeated to a minimum of 5 times by the alternative addition of Cu(2+) and S(2-), implying that FL is a renewable dual-functional chemosensor. The biocompatibility of FL toward breast carcinoma cells, MDA-MB-231 was confirmed by MTT assay. The reversible "ON-OFF-ON" fluorescent response of FL to Cu(2+) and S(2-) in living system was further confirmed by confocal fluorescence imaging of living cells. The quantification of Cu(2+) and S(2-) in single intact cell was realized by the flow cytometry analysis.