Use of Nuclear Magnetic Resonance (NMR) to Study Cancer Metabolism.

Nuclear magnetic resonance (NMR) spectroscopy has emerged as an important tool in cancer metabolism research, providing noninvasive, quantitative, and revealing insights into tumor biochemistry. This chapter discusses a wide range of NMR applications in oncology, including biofluid metabolomics for early diagnosis and prognosis, high-resolution magic angle spinning (HR-MAS) NMR for intact tissue profiling, and stable isotope-resolved metabolomics (SIRM) for mapping metabolic fluxes. Furthermore, the use of NMR in cancer cell culture models and the transformative influence of dynamic nuclear polarization (DNP)-enhanced NMR for real-time metabolic imaging are addressed. Collectively, these techniques allow for thorough analysis of metabolic reprogramming in cancer, biomarker development, and tailored therapy strategies.