Green Synthesis of Magnesium Nanoparticles From Native Neotropical Savannah Plants For Scalable Nanophytocosmetic Formulations.

This study describes a protocol for the green synthesis of magnesium-based nanoparticles using ethanol extracts of Stryphnodendron adstringens, a plant species native to the Neotropical savanna, and their incorporation into nanophytocosmetic formulations. The procedure includes collection and preparation of plant material, followed by ethanol extraction, in which phytochemicals act as reducing and stabilizing agents for magnesium ions. Nanoparticles are synthesized under controlled conditions using magnesium chloride and plant extracts, enabling an environmentally friendly process without the use of toxic reducing agents. Nanoparticle properties are characterized using dynamic light scattering and atomic force microscopy to assess size distribution, morphology, and colloidal behavior. The protocol further describes the incorporation of the synthesized nanoparticles into a cosmetic serum formulation. Stability is evaluated under controlled storage conditions by monitoring organoleptic properties, pH, and viscosity. This method provides a reproducible and sustainable approach for producing plant-mediated magnesium nanoparticles and assessing their application in cosmetic formulations.