Georgian medical news · 2026
COMPREHENSIVE ASSESSMENT OF BIOFILM FORMATION AND ANTIMICROBIAL RESISTANCE OF STAPHYLOCOCCUS IN PURULENT-INFLAMMATORY DISEASES.
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Rashova M, Kabduova A, Sailau Z, Serikberli G, Nurmukhamed K, Munaidarova A
Аннотация
INTRODUCTION: Treatment of biofilms is a priority in purulent surgery. A biofilm consists of 75-85% extracellular polymeric matrix and 15-20% microbial cells. Polysaccharides, proteins, and extracellular DNA within the matrix protect bacteria from adverse environmental factors (pH, antibiotics, phagocytosis). Communication between bacteria occurs through the "quorum sensing" system. Bacteria within biofilms are 100-1000 times more resistant to antibiotics compared to planktonic forms. This is explained by the limited penetration of antibiotics into the matrix and the reduced metabolic activity of the cells. Bacteria of the genus Staphylococcus, particularly Staphylococcus aureus, are currently recognized as the main causative agents of purulent-inflammatory diseases.
OBJECTIVE OF THE STUDY: to comprehensively assess the biofilm-forming activity of staphylococcal strains isolated from patients with purulent-inflammatory diseases of soft tissues (PIDs), as well as to determine the characteristics of their sensitivity to the main antimicrobial agents.
MATERIALS AND METHODS: To achieve the objectives of the study, we conducted a research project and examined 80 strains of the genus Staphylococcus isolated from purulent-inflammatory diseases. Of these, 50 belonged to the main group (MG) and 30 to the control group (CG). The identification of species characteristics of the 80 strains from the main and control groups was carried out based on their morphological, cultural, and biochemical properties. In addition, these strains were identified using MALDI-TOF mass spectrometry.
RESULTS: Analysis of the obtained results showed that S. aureus was predominant in both groups. During the study, 50 staphylococcal strains and two species-S. aureus and S. epidermidis-were identified among samples collected from patients with purulent-inflammatory diseases. In the control group, out of 30 strains, only one species-S. aureus-was identified. Biofilm-forming activity was also assessed based on microcolony size, and the morphological and tinctorial properties of isolated biofilm samples were studied. The field of view, number of objects, and their proportion within the field of view were calculated using digital images of the samples. The following microcolony sizes were taken into account: up to 10 µm², from 10 to 100 µm², from 100 to 1000 µm², from 1000 to 10,000 µm², and over 10,000 µm².
OBJECTIVE OF THE STUDY: to comprehensively assess the biofilm-forming activity of staphylococcal strains isolated from patients with purulent-inflammatory diseases of soft tissues (PIDs), as well as to determine the characteristics of their sensitivity to the main antimicrobial agents.
MATERIALS AND METHODS: To achieve the objectives of the study, we conducted a research project and examined 80 strains of the genus Staphylococcus isolated from purulent-inflammatory diseases. Of these, 50 belonged to the main group (MG) and 30 to the control group (CG). The identification of species characteristics of the 80 strains from the main and control groups was carried out based on their morphological, cultural, and biochemical properties. In addition, these strains were identified using MALDI-TOF mass spectrometry.
RESULTS: Analysis of the obtained results showed that S. aureus was predominant in both groups. During the study, 50 staphylococcal strains and two species-S. aureus and S. epidermidis-were identified among samples collected from patients with purulent-inflammatory diseases. In the control group, out of 30 strains, only one species-S. aureus-was identified. Biofilm-forming activity was also assessed based on microcolony size, and the morphological and tinctorial properties of isolated biofilm samples were studied. The field of view, number of objects, and their proportion within the field of view were calculated using digital images of the samples. The following microcolony sizes were taken into account: up to 10 µm², from 10 to 100 µm², from 100 to 1000 µm², from 1000 to 10,000 µm², and over 10,000 µm².