Clinical biochemistry · 2000
Transportation of maternal serum specimens for screening for chromosomal aneuploidies: effect of seasonal variations, distance, and freezing on the stability of the biological markers.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Massé J, Summers A, Cherian G, Forest J
Аннотация
OBJECTIVES: We studied the stability of maternal serum markers for screening for Down syndrome [alpha-fetoprotein (AFP), unconjugated estriol (uE3), total human chorionic gonadotropin (hCG), and free beta-human chorionic gonadotropin] following transportation.
DESIGN AND METHODS: For each gestational week between 14 and 18 weeks, we collected about 50 samples for each possible combination of three potential sources of variation during transportation: season (winter/summer), sample frozen/fresh (prior to analysis) and origin (local or referred in) for a total of 1845 specimens. Commercial assays were used (IMx, Abbott for AFP and total hCG; AutoDelfia and Wallac for free beta-hCG and AFP; and radioimmunoassay Ortho-Clinical Diagnostics, for uE3).
RESULTS: Results of both total and free beta-hCG were significantly higher when samples were collected during summer and when they were sent to the laboratory from extramural blood drawing sites (multifactorial ANOVA, p < 0. 007).
CONCLUSION: We conclude that both total and free beta-hCG can be adversely affected during transportation. The resulting higher concentrations of these markers may increase the false positive rate of prenatal screening programs for aneuploidies.
DESIGN AND METHODS: For each gestational week between 14 and 18 weeks, we collected about 50 samples for each possible combination of three potential sources of variation during transportation: season (winter/summer), sample frozen/fresh (prior to analysis) and origin (local or referred in) for a total of 1845 specimens. Commercial assays were used (IMx, Abbott for AFP and total hCG; AutoDelfia and Wallac for free beta-hCG and AFP; and radioimmunoassay Ortho-Clinical Diagnostics, for uE3).
RESULTS: Results of both total and free beta-hCG were significantly higher when samples were collected during summer and when they were sent to the laboratory from extramural blood drawing sites (multifactorial ANOVA, p < 0. 007).
CONCLUSION: We conclude that both total and free beta-hCG can be adversely affected during transportation. The resulting higher concentrations of these markers may increase the false positive rate of prenatal screening programs for aneuploidies.