Placenta · 2025
Exploring the role of specialized pro-resolving mediators, fatty acid oxidation markers and transporters in Gestational Diabetes Mellitus (GDM) placentae.
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Joshi Nikita, Sundrani Deepali, Pisal Hemlata, Randhir Karuna, Wagh Girija, Lalwani Sanjay, Gupte Sanjay, Joshi Sadhana
Аннотация
BACKGROUND: The role of fatty acids is critical throughout pregnancy and can influence pregnancy outcome. Materno-fetal transfer of fatty acids depends on placental fatty acid metabolism which includes fatty acid specialized pro-resolving mediators (SPMs) production, activation of peroxisome proliferated activated receptors (PPARs) and fatty acid oxidation. Alterations in any of these mechanisms may influence fetal development. This study reports placental levels of SPMs (protectins and lipoxins), PPARα, fatty acid oxidation marker (CPT1A) and fatty acid transporters (FATP1 and FATP4) in GDM and non-GDM women.
METHODS: The study included 209 women with GDM and 207 non-GDM women. The levels of protectins, lipoxins, PPARα, CPT1A, FATP1 and FATP4 were estimated using ELISA kits from placental tissue homogenates. The placental gene expression of PPARα, CPT1A, FATP1 and FATP4 was examined using quantitative real time PCR. Pearson's correlation was used to explore the association between these placental markers.
RESULTS: The placental protectin D1 (PD1; p < 0.05) was decreased whereas the lipoxin A4 (LXA4; p < 0.01), CPT1A, FATP1 and FATP4 (p < 0.05; for all) were increased in GDM as compared to non-GDM. The mRNA expression of PPARα, CPT1A, FATP1 and FATP4 were comparable between groups. The LXA4 and PD1 were positively correlated with PPARα (p < 0.01; for both). There was a positive association of PPARα with CPT1A, FATP1 and FATP4 (p < 0.01; for all).
CONCLUSION: Specialized pro-resolving mediators, fatty acid oxidation markers and fatty acid transporters are altered in GDM placentae.
METHODS: The study included 209 women with GDM and 207 non-GDM women. The levels of protectins, lipoxins, PPARα, CPT1A, FATP1 and FATP4 were estimated using ELISA kits from placental tissue homogenates. The placental gene expression of PPARα, CPT1A, FATP1 and FATP4 was examined using quantitative real time PCR. Pearson's correlation was used to explore the association between these placental markers.
RESULTS: The placental protectin D1 (PD1; p < 0.05) was decreased whereas the lipoxin A4 (LXA4; p < 0.01), CPT1A, FATP1 and FATP4 (p < 0.05; for all) were increased in GDM as compared to non-GDM. The mRNA expression of PPARα, CPT1A, FATP1 and FATP4 were comparable between groups. The LXA4 and PD1 were positively correlated with PPARα (p < 0.01; for both). There was a positive association of PPARα with CPT1A, FATP1 and FATP4 (p < 0.01; for all).
CONCLUSION: Specialized pro-resolving mediators, fatty acid oxidation markers and fatty acid transporters are altered in GDM placentae.