Effects of neonatal C-fiber depletion on neocortical long-term potentiation and depression.

Capsaicin (Cap)-induced depletion of C-fiber afferents results in plasticity of somatosensory system which is manifested as a functional alteration at different levels of the somatosensory pathway. In the present study we examined the effect of Cap-induced neonatal depletion of C-fibers on the induction of long-term potentiation (LTP) and long-term depression (LTD) in the neocortex of freely moving rats. A stimulating electrode was implanted into corpus callosum and a recording electrode was implanted in the somatosensory cortex of control (Con: normal, without electrical stimulation), trained (normal, with electrical stimulation) and Cap-treated (C-fiber depleted, with electrical stimulation) adult rats. Two weeks after the surgery, evoked field potential responses were recorded before, during (12 days) and after (1 month) the induction period of LTP and LTD. The LTP and LTD response characteristics during the time course of recording were compared between different groups. In the train group, LTP and LTD appeared after 3 days of stimulation. LTP magnitude peaked after about 6 days while LTD magnitude peaked in about 12 days. C-fiber depletion postponed the development of LTP and LTD making the highest differential levels of LTP about 6 days after the initiation of LTP induction. The impact of C-fiber depletion on slowing the time course of LTD induction was more prolonged and lasted until day 12 of the initiation of LTD induction. These results suggest that intact C-fibers are necessary for normal plasticity and long-term synaptic modification of the somatosensory system.