Медицинская библиотека · Metabolomics : Official journal of the Metabolomic Society · 2025

Feasibility of amino acid profiling in long-term stored formalin-fixed paraffin-embedded colorectal neoplasia tissue.

Opperman Roza C M, Bruchner Puck E, Bosch Sofie, de Meij Tim G J, Dekker Evelien, de Boer Nanne K H, Struys Eduard A

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Аннотация

INTRODUCTION

Metabolic processes play a role in cancer development, with faecal amino acids emerging as potential biomarkers for colorectal neoplasia. While fresh frozen tissue is preferred for metabolomic analysis, formalin-fixed paraffin-embedded (FFPE) tissue is more widely available.

OBJECTIVES

We aimed to evaluate amino acid profiles in FFPE tissue across different stages of the adenoma-carcinoma sequence.

METHOD: A panel of 20 amino acids was measured using liquid chromatography-tandem mass spectrometry.

RESULTS

Fourteen amino acids were detected, with proline elevated in colorectal carcinoma versus advanced (FC 2.33, p = 0.04) and non-advanced adenomas (FC 2.42, p = 0.02).

CONCLUSION

Despite analytical challenges, amino acid profiling in FFPE tissue is feasible.

Для цитирования: Opperman Roza C M, Bruchner Puck E, Bosch Sofie, de Meij Tim G J, Dekker Evelien, de Boer Nanne K H, Struys Eduard A. Feasibility of amino acid profiling in long-term stored formalin-fixed paraffin-embedded colorectal neoplasia tissue.. Metabolomics : Official journal of the Metabolomic Society. 2025. DOI: 10.1007/s11306-025-02301-8.

Предметные рубрики MeSH

Humans Colorectal Neoplasms Amino Acids Paraffin Embedding Formaldehyde Tandem Mass Spectrometry Chromatography, Liquid Tissue Fixation Adenoma Feasibility Studies Metabolomics Male Biomarkers, Tumor Female Middle Aged Aged

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