Investigations of Cu2ONPs - Orange Peels: Biosynthesis, Characterization, and Anticancer Activity on a Cytotoxic Breast Cancer Cell Line.

BACKGROUND

The study aimed to evaluate the efficacy of nanocomposites extracted from orange peels in targeting and combating MCF-7 cells.

METHODS

We characterized the nanoparticles using XRD, FTIR, SEM, and GC-MS, and evaluated their cytotoxicity and activity using the MTT colorimetric assay and double staining technique.

RESULTS

The obtained results showed that the nanoparticles ranged from 19 to 66 nanometers in size and contained active compounds in the range of 4,000-500 cm⁻¹. However, the XRD sizes of the crystalline nanoparticles ranged from 15 to 23 nm. Orange peel contains important compounds such as octanal, 1, 6-octadien-3-ol, 3, 7-dimethyl, and limonene. At 800 mg/ml, DPPH can act as a strong antioxidant and reduce free radicals by 78.29%. The addition of the molecules resulted in a trace amount of 3.99%, a viability of 74.671%, and a half-maximal inhibitory concentration of 43.75 g/mL for the cell line. The compatibility with the low concentration effectively repaired the damaged cells.

CONCLUSIONS

We expect the present study to provide important insights into the influence of molecular structure on anti-cancer action.