Frontiers in microbiology · 2026
CSF peptidomics reveals an IGF2-derived peptide as a candidate biomarker for neurosyphilis diagnosis.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Yin Yue, Li Xinru, Han Jiayin, Li Jiatong, Zeng Lijiao, Lan Jianfeng, Weng Weizhen, Liang Yanhua, Zhang Ying, Zou Rongrong, Wu Shuai, Tan Guoyu, Tang Shenghong, Yuan Jing
Аннотация
BACKGROUND: Neurosyphilis (NS) diagnosis is challenging due to the cerebrospinal fluid-Venereal Disease Research Laboratory (CSF-VDRL) assay has limited sensitivity, whereas CSF abnormalities lack sufficient specificity.
METHODS: To identify novel CSF peptide biomarkers for NS, we performed comparative peptidomic analysis of CSF samples from 51 individuals across four groups: NS, syphilis/non-NS (NNS), infectious brain diseases without syphilis (IBD), and non-infectious brain disorders without syphilis (NIBD). Peptides were profiled by high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS). Bioinformatic analysis and machine learning identified candidate biomarkers, validated by parallel reaction monitoring (PRM) in an independent cohort (n = 23).
RESULTS: A total of 4,738 peptides were identified. The insulin-like growth factor 2 (IGF2)-derived peptide GHVLAKELE showed strong discriminative performance: area under the curve (AUC) values of 0.914 (NS vs. NNS), 0.964 (NS vs. IBD), and 0.878 (NS vs. NIBD).
CONCLUSION: This first systematic CSF peptidomic characterization of neurosyphilis identifies GHVLAKELE as a promising diagnostic biomarker.
METHODS: To identify novel CSF peptide biomarkers for NS, we performed comparative peptidomic analysis of CSF samples from 51 individuals across four groups: NS, syphilis/non-NS (NNS), infectious brain diseases without syphilis (IBD), and non-infectious brain disorders without syphilis (NIBD). Peptides were profiled by high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS). Bioinformatic analysis and machine learning identified candidate biomarkers, validated by parallel reaction monitoring (PRM) in an independent cohort (n = 23).
RESULTS: A total of 4,738 peptides were identified. The insulin-like growth factor 2 (IGF2)-derived peptide GHVLAKELE showed strong discriminative performance: area under the curve (AUC) values of 0.914 (NS vs. NNS), 0.964 (NS vs. IBD), and 0.878 (NS vs. NIBD).
CONCLUSION: This first systematic CSF peptidomic characterization of neurosyphilis identifies GHVLAKELE as a promising diagnostic biomarker.