Chemistry & biodiversity · 2025
Anxiolytic Effects of Indole Alkaloids From Rauvolfia ligustrina in Adult Zebrafish: Involvement of the GABAergic and 5-HT Systems.
СтатьяНазвание и аннотация: Перевод в очереди
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Pinheiro Nádia Aguiar Portela, Romão Ivana Carneiro, Alves Amanda Maria Barros, Siqueira Sônia Maria Costa, de Menezes Jane Eire Silva Alencar, Marinho Emmanuel Silva, Marinho Márcia Machado, Magalhães Herbert de Sousa, Gomes Andreia Ferreira de Castro, Pessoa Otília Deusdênia Loiola, Dos Santos Hélcio Silva
Аннотация
Benzodiazepines are widely used in the treatment of anxiety, but their use may be interrupted due to side effects. This study evaluated the indole alkaloids isocarapanaubine (indole alkaloid 1 [AIN1]) and 18-β-hydroxy-3-epi-α-yohimbine (indole alkaloid 2 [AIN2]), extracted from Rauvolfia ligustrina, in adult zebrafish. Three doses (4, 12, and 20 mg/kg) were tested, along with dimethyl sulfoxide (DMSO) 3% and Diazepam (4 mg/kg) in toxicity, locomotion, and anxiolytic effect assays. AIN1 and AIN2 showed low toxicity (lethal dose capable of killing 50% of the animals >20 mg/kg) and reduced locomotor activity. Both exhibited anxiolytic effects, with AIN1 acting through the GABAergic system and AIN2 via the serotonergic pathway (5-HT3A). Interactions with receptors were confirmed by molecular docking, and drug metabolism and pharmacokinetics studies indicated that AIN2 is promising in terms of permeability and safety in the central nervous system.