Behavioral Responses of the Bumblebee Bombus terrestris to Volatile Compounds from Blueberries.

Bumblebees are important pollinating insects in crop pollination. Chemical attractants can effectively improve the flower-visiting efficiency of bumblebees, thereby increasing blueberry yields. To identify volatile compounds that attract bumblebees, we collected volatile compounds from blueberry flowers using headspace extraction. Gas chromatography- mass spectrometry (GC-MS) identified 32 volatile compounds, with Linalool and Styrene being the primary substances that accounted for 25.93% and 14.28%, respectively. The olfactory threshold of bumblebee antennae was assessed using electroantennography (EAG), and the behavioral responses from bumblebees were investigated using a Y-tube olfactometer. Results indicate that among the six classes of volatiles-alcohols, aldehydes, esters, ketones, aromatic compounds, and olefins-alcohols constituted the predominant proportion. Among these, six compounds-benzaldehyde, phenylpropylaldehyde, citral, linalool, α-terpineol, and geraniol-induced significant antennal responses in bumblebees. Our assays showed that geraniol, linalool, and α-terpineol at concentrations of 0.1 μg/μL, 1 μg/μL, and 10 μg/μL elicited attraction, whereas higher concentrations of benzaldehyde, benzenepropanal, and citral had repellent effects.