Zhonghua yi xue za zhi · 2011
[Effects of EMPs on HUVECs' functions in vitro].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Zheng Min-Zhe, Wu Zhi-Hong, Ji Chun-Liang, Li Hui, Qiu Gui-Xing, Weng Xi-Sheng
Аннотация
OBJECTIVE: To investigate the effects of EMPs on HUVECs permeability in vitro, then evaluate the effect on the Avascular Necrosis of Femoral Head.
METHODS: Incubate HUVECs with EMPs which is released by HUVECs in vitro, then estimate the endothelial permeability.
RESULTS: Flow cytometry analysis showed that EMPs were released at maximum concentration(7.66 × 10(4)/ml) after 24 hours stimulated by 10 mmol DEX And 7.66 × 10(4), 7.66 × 10(3)/ml concentration (moderate and high concentrations) of EMPs caused injury to the permeability of HUVECs (P values are 0.002 and 0.031 respectively, < 0.05), while 7.66 × 10(2)/ml concentration (low concentration) of EMPs didn't (P = 0.425 > 0.05).
CONCLUSION: Dexamethasone can induce HUVECs to release EMPs in vitro. EMPs caused collapse of connections of HUVECs, and increase the HUVECs permeability, decrease vascular stability, finally lead to Avascular Necrosis of Femoral Head.
METHODS: Incubate HUVECs with EMPs which is released by HUVECs in vitro, then estimate the endothelial permeability.
RESULTS: Flow cytometry analysis showed that EMPs were released at maximum concentration(7.66 × 10(4)/ml) after 24 hours stimulated by 10 mmol DEX And 7.66 × 10(4), 7.66 × 10(3)/ml concentration (moderate and high concentrations) of EMPs caused injury to the permeability of HUVECs (P values are 0.002 and 0.031 respectively, < 0.05), while 7.66 × 10(2)/ml concentration (low concentration) of EMPs didn't (P = 0.425 > 0.05).
CONCLUSION: Dexamethasone can induce HUVECs to release EMPs in vitro. EMPs caused collapse of connections of HUVECs, and increase the HUVECs permeability, decrease vascular stability, finally lead to Avascular Necrosis of Femoral Head.