Research in pharmaceutical sciences · 2025
Nephroprotective effects of ethanol leaf extract of Stachys pilifera Benth in alcohol-induced nephrotoxicity in male rats.
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Mirghaed Ali Taheri, Khoshkharam Narsis, Karimi Farzaneh, Abdzadeh Soroor, Razazan Marzieh, Rahami Zahra, Doustimotlagh Amir Hossein
Аннотация
BACKGROUND AND PURPOSE: Excessive and long-term consumption of alcohol causes health problems and significant changes in the physiological and biochemical functions of the body organs, including the kidneys. The present study aimed to clarify and investigate the antioxidant and anti-inflammatory impacts of Stachys pilifera Benth (SPB) hydroalcoholic extract on ethanol (EtOH)-induced nephrotoxicity.
EXPERIMENTAL APPROACH: Twenty-four rats were randomly divided into 4 groups. The control group was administered distilled water as a vehicle for 35 days. The EtOH group received ethanol (40% v/v, 7 mL/kg) orally for 35 days. The SPB extract + EtOH group was pretreated with 500 mg/kg of SPB orally and, after 1 h, received ethanol (40% v/v, 7 mL/kg) orally for 35 days; the SPB extract group received only 500 mg/kg of SPB for 35 days. After 35 days, the rats were sacrificed, and the blood sample was taken. Then, levels of biochemical markers, oxidative stress indices, antioxidant enzyme activity, and pro-inflammatory cytokines were evaluated.
FINDINGS/RESULTS: In the EtOH group, the levels of BUN, Cr, NO metabolite, and MDA were significantly higher compared to the control group. In addition, a significant elevation in the levels of pro-inflammatory cytokines (TNF-α and IL-6) was observed compared to the control group. Pretreatment with SPB hydroalcoholic extract led to significant diminishment in BUN, Cr, MDA, NO metabolite, and TNF-α and IL-6.
CONCLUSION AND IMPLICATIONS: The hydroalcoholic extract of SPB led to improvement of kidney function, reduction of oxidative stress, and improvement of inflammatory conditions in nephrotoxicity caused by ethanol in male rats.
EXPERIMENTAL APPROACH: Twenty-four rats were randomly divided into 4 groups. The control group was administered distilled water as a vehicle for 35 days. The EtOH group received ethanol (40% v/v, 7 mL/kg) orally for 35 days. The SPB extract + EtOH group was pretreated with 500 mg/kg of SPB orally and, after 1 h, received ethanol (40% v/v, 7 mL/kg) orally for 35 days; the SPB extract group received only 500 mg/kg of SPB for 35 days. After 35 days, the rats were sacrificed, and the blood sample was taken. Then, levels of biochemical markers, oxidative stress indices, antioxidant enzyme activity, and pro-inflammatory cytokines were evaluated.
FINDINGS/RESULTS: In the EtOH group, the levels of BUN, Cr, NO metabolite, and MDA were significantly higher compared to the control group. In addition, a significant elevation in the levels of pro-inflammatory cytokines (TNF-α and IL-6) was observed compared to the control group. Pretreatment with SPB hydroalcoholic extract led to significant diminishment in BUN, Cr, MDA, NO metabolite, and TNF-α and IL-6.
CONCLUSION AND IMPLICATIONS: The hydroalcoholic extract of SPB led to improvement of kidney function, reduction of oxidative stress, and improvement of inflammatory conditions in nephrotoxicity caused by ethanol in male rats.