Effects of flotation reagents with aniline aerofloat and ammonium dibutyl dithiophosphate on a constructed rapid infiltration system: Performance and microbial metabolic pathways.

Aniline aerofloat (AAF) and ammonium dibutyl dithiophosphate (ADD) are the key flotation reagents in mineral processing. This study investigated the performance of the constructed rapid infiltration systems with coke and red mud as adsorbents for treatment AAF and ADD wastewater. Meanwhile, the effects of AAF and ADD on the microbial metabolic pathways of the systems were unraveled. Results showed that the AAF concentration in influent was 25 mg/L, which promoted chemical oxygen demand (COD) and total phosphorus (TP) removal of the A column (coke) and B column (red mud). While the COD and TP removal of the C column (coke) and D column (red mud) were inhibited with ADD concentration increasing to 50 mg/L and 100 mg/L. The AAF reduced the binding energy of coke C-O bond by 0.9 eV, and down-regulated the C-C bond ratio by 40.72%. The dominant phyla in the columns were Pseudomonadota and Actinomycetota. The pore structure of coke was more conducive to the growth of the Pseudomonadota, while the metal composition of red mud was more conducive to the redox reaction of microorganisms. The presence of phosphofructokinase (2.7.1.11)-related genes was up-regulated in column C compared to other columns. The ADD was beneficial to the expression of norC and nosZ functional genes during nitrogen metabolism process. In contrast, phosphorus metabolism genes were more expressed in the red mud column for treatment AAF wastewater. This study reveals the potential of coke and red mud for the treatment of flotation reagents wastewater, while providing a theoretical basis for the optimal selection of filler types in the constructed rapid infiltration systems.