BJU international · 2008
A functional study of purinergic signalling in the normal and pathological rabbit corpus cavernosum.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Calvert Robert C, Khan Masood A, Thompson Cecil S, Mikhailidis Dimitri P, Burnstock Geoffrey
Аннотация
OBJECTIVE: To examine rabbit cavernosal smooth muscle (CSM) relaxation to ATP, ADP and UTP in normal rabbits and in models of conditions that predispose to erectile dysfunction (ED), diabetes mellitus (DM; induced for 6 months) and bladder outlet obstruction (BOO, 6 weeks after surgery).
MATERIALS AND METHODS: Concentration-response curves (CRCs) were constructed to ATP, ADP and UTP on CSM from control rabbits in the absence and presence of antagonists. In addition, CRCs were constructed to ATP in CSM from rabbits with DM and BOO.
RESULTS: ATP and UTP caused equipotent, dose-dependent relaxations of pre-contracted normal rabbit CSM; ADP was more potent. Relaxation was inhibited by Reactive Blue 2, but not by suramin, 8-p-sulfophenyltheophylline or L-N(G)-nitroarginine methyl ester. In rabbits with DM and those with partial BOO, ATP-mediated CSM relaxation was less than in control rabbits. Pharmacological profiling suggests that purine-induced CSM relaxation might be mediated by P2Y(1) and P2Y(4) receptors in the rabbit.
CONCLUSIONS: In healthy rabbits, ATP released from nerves appears to produce relaxation of CSM via P2Y(4) receptors on smooth muscle, while ADP, acting on P2Y(1) receptors on endothelial cells, produces relaxation via nitric oxide. Alterations in CSM purinergic signalling might be implicated in the pathophysiology of ED associated with DM and BOO. Characterization of purinergic signalling in CSM might highlight new therapeutic targets for treating ED.
MATERIALS AND METHODS: Concentration-response curves (CRCs) were constructed to ATP, ADP and UTP on CSM from control rabbits in the absence and presence of antagonists. In addition, CRCs were constructed to ATP in CSM from rabbits with DM and BOO.
RESULTS: ATP and UTP caused equipotent, dose-dependent relaxations of pre-contracted normal rabbit CSM; ADP was more potent. Relaxation was inhibited by Reactive Blue 2, but not by suramin, 8-p-sulfophenyltheophylline or L-N(G)-nitroarginine methyl ester. In rabbits with DM and those with partial BOO, ATP-mediated CSM relaxation was less than in control rabbits. Pharmacological profiling suggests that purine-induced CSM relaxation might be mediated by P2Y(1) and P2Y(4) receptors in the rabbit.
CONCLUSIONS: In healthy rabbits, ATP released from nerves appears to produce relaxation of CSM via P2Y(4) receptors on smooth muscle, while ADP, acting on P2Y(1) receptors on endothelial cells, produces relaxation via nitric oxide. Alterations in CSM purinergic signalling might be implicated in the pathophysiology of ED associated with DM and BOO. Characterization of purinergic signalling in CSM might highlight new therapeutic targets for treating ED.