Journal of endodontics · 2026
Early Pulp Healing After Direct Pulp Capping with Concentrated Platelet-Rich Fibrin: A Preliminary Study.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zhang Xingyu, Chai Jihua, Miron Richard J, Wang Yulan, Yu Shimin, Shi Shan, Li Sensen, Tong Guoyong, Yang Zaibo, Chen Meiling
Аннотация
BACKGROUND: Direct pulp capping preserves pulp vitality after pulp exposure. Mineral trioxide aggregate (MTA) is widely used but relies mainly on host-mediated repair. Concentrated platelet-rich fibrin (C-PRF), an autologous plasma-derived biomaterial, may actively modulate the local healing microenvironment through sustained delivery of bioactive factors, but its biological performance in direct pulp capping has not been fully characterized.
METHODS: Human dental pulp cells were treated with phosphate-buffered saline, MTA, or C-PRF to assess viability, proliferation, migration, and mineralization via live/dead staining, CCK-8, scratch and transwell assays, alkaline phosphatase and Alizarin Red S staining, as well as qPCR for genes encoding alkaline phosphatase, RUNX2, and COL1A1. Furthermore, an in vivo rabbit direct pulp capping model was utilized and treated with MTA or C-PRF. Pulp regenerative potential was evaluated using hematoxylin and eosin, Masson's trichrome, and CD31 immunohistochemistry, with quantitative analysis of vascularization.
RESULTS: C-PRF demonstrated good biocompatibility and enhanced human dental pulp cells migration, proliferation, and mineralization compared with MTA. In vivo, the C-PRF group exhibited increased CD31-positive staining, suggesting enhanced early vascular responses.
CONCLUSION: Within the limitations of this study, C-PRF enhanced cellular responses, including migration and mineralization, and showed potential to promote early vascularization. These findings suggest that C-PRF may serve as a biologically active adjunct for direct pulp capping.
METHODS: Human dental pulp cells were treated with phosphate-buffered saline, MTA, or C-PRF to assess viability, proliferation, migration, and mineralization via live/dead staining, CCK-8, scratch and transwell assays, alkaline phosphatase and Alizarin Red S staining, as well as qPCR for genes encoding alkaline phosphatase, RUNX2, and COL1A1. Furthermore, an in vivo rabbit direct pulp capping model was utilized and treated with MTA or C-PRF. Pulp regenerative potential was evaluated using hematoxylin and eosin, Masson's trichrome, and CD31 immunohistochemistry, with quantitative analysis of vascularization.
RESULTS: C-PRF demonstrated good biocompatibility and enhanced human dental pulp cells migration, proliferation, and mineralization compared with MTA. In vivo, the C-PRF group exhibited increased CD31-positive staining, suggesting enhanced early vascular responses.
CONCLUSION: Within the limitations of this study, C-PRF enhanced cellular responses, including migration and mineralization, and showed potential to promote early vascularization. These findings suggest that C-PRF may serve as a biologically active adjunct for direct pulp capping.