Archives of biochemistry and biophysics · 2001
Tamulustoxin: a novel potassium channel blocker from the venom of the Indian red scorpion Mesobuthus tamulus.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Strong P N, Clark G S, Armugam A, De-Allie F A, Joseph J S, Yemul V, Deshpande J M, Kamat R, Gadre S V, Gopalakrishnakone P, Kini R M, Owen D G, Jeyaseelan K
Аннотация
We have characterized tamulustoxin, a novel 35-amino-acid peptide found in the venom of the Indian red scorpion (Mesobuthus tamulus). Tamulustoxin was identified through a [125I]toxin I screen, designed to identify toxins that block voltage-activated potassium channels. Tamulustoxin has also been cloned by RT-PCR, using RNA extracted from scorpion venom glands. Tamulustoxin shares no homology with other scorpion venom toxins, although the positions of its six cysteine residues would suggest that it shares the same structural scaffold. Tamulustoxin rapidly inhibited both peak and steady-state currents (18.9 +/- 1.0 and 37 +/- 1.1%, respectively) produced by injecting CHO cells with mRNA encoding the hKv1.6 channel.
Предметные рубрики MeSH
Amino Acid SequenceAnimalsBase SequenceCHO CellsCalciumChromatography, AgaroseCloning, MolecularCricetinaeCysteineDNA, ComplementaryGas Chromatography-Mass SpectrometryMolecular Sequence DataPatch-Clamp TechniquesPeptidesPotassium Channel BlockersPotassium ChannelsProtein BindingProtein ConformationRNARNA, MessengerReverse Transcriptase Polymerase Chain ReactionScorpion VenomsScorpionsSequence Homology, Amino Acid