The Journal of reproductive medicine · 2008
Interleukin-18 gene promoter polymorphisms in women with gestational trophoblastic diseases.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Kashef Mohammad Amin, Dehaghani Alamtaj Samsami, Naeimi Sirous, Fattahi Mohammad Javad, Ghaderi Abbas
Аннотация
OBJECTIVE: Gestational trophoblastic diseases (GTDs) consist of a spectrum of disorders characterized by an abnormal proliferation of trophoblastic tissue. IL-18 is a pleiotropic cytokine with a capacity for both ThI and Th2 polarization. Considering the association of IL-18 promoter polymorphisms at positions -607 (A/C) and -137 (C/G) with pregnancy events and some cancers, we sought to examine these polymorphisms in Iranian patients with GTD, their association with disease subtypes, and IL-18 serum level.
STUDY DESIGN: Single nucleotide polymorphisms (SNPs) were analyzed by allele-specific polymerase chain reaction in 92 patients with GTDs and 103 healthy pregnant controls. IL-18 serum level was determined using ELISA method.
RESULTS: No significant association was found between the allele, genotype, genotype combination and haplotype distribution of these SNPs and GTDs or its subgroups. Mean IL-18 serum level was significantly higher in patients with choriocarcinoma and pregnant controls compared with nonpregnant controls (p = 0.04, 0.04 and 0.001, respectively). -137 GG genotype pregnant controls had a significantly higher IL-18 serum level compared with CC genotype.
CONCLUSION: IL-18 promoter polymorphisms do not confer susceptibility to GTDs or its variants; however, their functional significance is demonstrated in this study. Furthermore, IL-18 serum level increases in GTDs and in normal pregnancy.
STUDY DESIGN: Single nucleotide polymorphisms (SNPs) were analyzed by allele-specific polymerase chain reaction in 92 patients with GTDs and 103 healthy pregnant controls. IL-18 serum level was determined using ELISA method.
RESULTS: No significant association was found between the allele, genotype, genotype combination and haplotype distribution of these SNPs and GTDs or its subgroups. Mean IL-18 serum level was significantly higher in patients with choriocarcinoma and pregnant controls compared with nonpregnant controls (p = 0.04, 0.04 and 0.001, respectively). -137 GG genotype pregnant controls had a significantly higher IL-18 serum level compared with CC genotype.
CONCLUSION: IL-18 promoter polymorphisms do not confer susceptibility to GTDs or its variants; however, their functional significance is demonstrated in this study. Furthermore, IL-18 serum level increases in GTDs and in normal pregnancy.