In vitro assessment of the immobilized mannanase enzyme against infection-causing Candida.

Background

Developing an effective treatment for fungal infections is among contemporary medicine's challenges. In this study we aimed to eradicate mannan in pathogenic Candidae's cell walls using a nontoxic method, mannanase. Materials &

Methods

We investigated the in vitro antifungal activities of mannanase immobilized on zinc oxide nanoparticles (ZnONps) and reduced graphene oxide nanoparticles (RGONps), as well as therapeutics against Candidae. Mannanase was purified from Bacillus invictae (activity: 5.15 EU/ml; range: 60-80%) and then immobilized it to ZnO and RGO to enhance its effectiveness.

Results

Mannanase immobilized on ZnONps had the highest activity, with a value of 4.97 EU/ml, more effective than amphotericin-B. However, it could not reach the inhibition rates of other antifungals.

Conclusion

Mannanase immobilized on ZnONps could be an effective fungicide for Candida biocontrol.