Synthesis, optimization, and characterization of biogenic manganese oxide (BioMnOx) by bacterial isolates from mangrove soils with sorbents property towards different toxic metals.

Manganese oxidizing bacteria, Bacillus mycoides and Bacillus subtilis were isolated from mangrove soils and optimized for the removal of Mn(II) with simultaneous production of biogenic manganese oxide (BioMnOx). The removal rate of Mn(II) was 90% in 48 h for B. mycoides and 72 h for B. subtilis under the optimized conditions at pH 7, temperature 37 °C, 120 rpm, with 1% inoculum containing 10 mM MnCl2. Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), Energy dispersive X-Ray analysis (EDAX), Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) were used to characterize the synthesized biogenic manganese oxide. BioMnOx by Bacillus mycoides and Bacillus subtilis were identified as Bixbyite (Mn2O3) and Hausmannite (Mn3O4), respectively, with nano-sized monocrystalline nature. BioMnOx of Bacillus subtilis strain was more efficient in the removal of metals Zn and Co than BioMnOx of Bacillus mycoides except for mercury. The removal property of synthesized BioMnOx could be applied to treat multi-metal containing wastewater.