Frontiers in veterinary science · 2026
A novel phage vB_VhaM_HVS-1 inhibits host cell associated Vibrio harveyi.
СтатьяНазвание и аннотация: Перевод в очереди
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Chen Qun, Ye Peipei, Pan Jieting, Chen Lin, Liu Xibang, Ding Lijian, Jiang Liming
Аннотация
INTRODUCTION: Vibrio harveyi is a major pathogen causing skin ulceration syndrome and mass mortality in sea cucumber Apostichopus japonicus aquaculture.
METHODS: In this study, a pathogenic strain NB-3 was isolated from diseased A. japonicus and identified as V. harveyi based on morphological, physiological, and genomic analyses. A novel lytic phage vB_VhaM_HVS-1 belonging to the family Straboviridae (class Caudoviricetes) was isolated from A. japonicus breeding pond silt.
RESULTS: The phage exhibited a latent period of 50 min and a burst size of approximately 168 PFU/cell, and its genome lacked lysogeny-related genes, suggesting a lytic nature. Phage treatment significantly reduced the number of cell-associated bacteria, decreasing the association rate from 2.3% to nearly 0. Flow cytometry revealed that phage infection protected coelomocytes from V. harveyi-induced damage, increasing the proportion of viable cells from 78.17% to 88.54%.
CONCLUSION: These findings demonstrate that phage vB_VhaM_HVS-1 effectively reduces V. harveyi association with coelomocytes and alleviates host cell apoptosis. The phage shows strong potential as an alternative biocontrol agent against V. harveyi infection in sea cucumber aquaculture.
METHODS: In this study, a pathogenic strain NB-3 was isolated from diseased A. japonicus and identified as V. harveyi based on morphological, physiological, and genomic analyses. A novel lytic phage vB_VhaM_HVS-1 belonging to the family Straboviridae (class Caudoviricetes) was isolated from A. japonicus breeding pond silt.
RESULTS: The phage exhibited a latent period of 50 min and a burst size of approximately 168 PFU/cell, and its genome lacked lysogeny-related genes, suggesting a lytic nature. Phage treatment significantly reduced the number of cell-associated bacteria, decreasing the association rate from 2.3% to nearly 0. Flow cytometry revealed that phage infection protected coelomocytes from V. harveyi-induced damage, increasing the proportion of viable cells from 78.17% to 88.54%.
CONCLUSION: These findings demonstrate that phage vB_VhaM_HVS-1 effectively reduces V. harveyi association with coelomocytes and alleviates host cell apoptosis. The phage shows strong potential as an alternative biocontrol agent against V. harveyi infection in sea cucumber aquaculture.