Veterinary medicine international · 2026
Carbapenemase Genes Surveillance in Pseudomonas aeruginosa From Domestic Animals: A Five-Year Study From Brazil's Midwest Region.
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Kawasaki Mayara Lima, Costa Jackeliny Dos Santos, Vieira Ester Santa, Silva Anna Caroline Barbosa Alves, Vani Camila Regina Alves, Caldeira Ana Carolina Lemos, Araujo Ana Rebecca Maia, Nakazato Luciano, Dutra Valeria
Аннотация
BACKGROUND: Pseudomonas aeruginosa represents a critical antimicrobial resistance (AMR) threat in veterinary medicine, with carbapenemase-producing isolates posing significant challenges to both animal and human health. The emergence of metallo-beta-lactamases (MBLs) and serine carbapenemases in veterinary settings necessitates comprehensive surveillance to monitor resistance gene dissemination.
OBJECTIVE: This study aimed to conduct genotypic surveillance of carbapenemase-encoding genes (blaKPC-2, blaSPM-1, blaNDM-1, blaVIM-2, and blaIMP-1) in P. aeruginosa isolates from domestic animals in Brazil's Midwest region, providing critical epidemiological data for antimicrobial stewardship programs.
METHODS: A total of 83 P. aeruginosa isolates were collected from various domestic animal species (57 canines, 12 bovines, 7 felines, 2 equines, 2 ovines, 2 avians, and 1 rabbit) between 2017 and 2021. Molecular identification was confirmed via 16S rRNA gene sequencing, and carbapenemase genes were detected using conventional multiplex PCR. Data were analyzed using descriptive statistics.
RESULTS: Carbapenemase genes were detected in 40.96% (34/83) of isolates. The most prevalent gene was blaKPC-2 at 34.9% (29/83), followed by blaSPM-1 (9.6%, 8/83), blaNDM-1 (8.4%, 7/83), blaVIM-2 (3.6%, 3/83), and blaIMP-1 (1.2%, 1/83). Ear infections were the predominant source (53%, 44/83), particularly in canines. Co-occurrence of multiple resistance genes was observed in 7.2% of isolates.
CONCLUSION: This study reveals an alarming prevalence of carbapenemase-producing P. aeruginosa in domestic animals, with blaKPC-2 frequencies comparable to those in wild animal populations. The predominance of ear-derived isolates suggests a potential for community-acquired transmission. These findings underscore the critical need for robust antimicrobial stewardship and integrated One Health surveillance systems to monitor resistance gene flow between animal and human populations.
OBJECTIVE: This study aimed to conduct genotypic surveillance of carbapenemase-encoding genes (blaKPC-2, blaSPM-1, blaNDM-1, blaVIM-2, and blaIMP-1) in P. aeruginosa isolates from domestic animals in Brazil's Midwest region, providing critical epidemiological data for antimicrobial stewardship programs.
METHODS: A total of 83 P. aeruginosa isolates were collected from various domestic animal species (57 canines, 12 bovines, 7 felines, 2 equines, 2 ovines, 2 avians, and 1 rabbit) between 2017 and 2021. Molecular identification was confirmed via 16S rRNA gene sequencing, and carbapenemase genes were detected using conventional multiplex PCR. Data were analyzed using descriptive statistics.
RESULTS: Carbapenemase genes were detected in 40.96% (34/83) of isolates. The most prevalent gene was blaKPC-2 at 34.9% (29/83), followed by blaSPM-1 (9.6%, 8/83), blaNDM-1 (8.4%, 7/83), blaVIM-2 (3.6%, 3/83), and blaIMP-1 (1.2%, 1/83). Ear infections were the predominant source (53%, 44/83), particularly in canines. Co-occurrence of multiple resistance genes was observed in 7.2% of isolates.
CONCLUSION: This study reveals an alarming prevalence of carbapenemase-producing P. aeruginosa in domestic animals, with blaKPC-2 frequencies comparable to those in wild animal populations. The predominance of ear-derived isolates suggests a potential for community-acquired transmission. These findings underscore the critical need for robust antimicrobial stewardship and integrated One Health surveillance systems to monitor resistance gene flow between animal and human populations.