Conformational effects on iodide binding: a comparative study of flexible and rigid carbazole macrocyclic analogs.

To our knowledge, this work represents one of the earliest comparative studies on the anion-binding behaviors of carbazole-based structural analogs, demonstrating that a flexible macrocycle markedly improves iodide binding affinity via an induced-fit mechanism. The flexible analog PBG exhibits a 22.78-fold higher fluorescence quenching efficiency upon iodide binding compared to the rigid WDG (K PBG /K WDG = 22.78), demonstrating its potential as a highly sensitive optical probe and offering a novel strategy for engineering dynamic supramolecular receptors. Two carbazole-based macrocyclic probes, PBG (flexible benzene ring) and WDG (rigid fluorene backbone), were synthesized via Friedel-Crafts reactions. Their iodide (I-) recognition properties were systematically explored using 1H NMR, UV-vis absorption, and fluorescence spectroscopy. Quantitative analysis via the Benesi-Hildebrand equation and nonlinear fitting demonstrated that flexible PBG achieves superior I- binding (K PBG = 1.387 × 105 M-1) through induced-fit conformational adjustments, whereas rigid WDG (K WDG = 6.089 × 103 M-1) is constrained by preorganized cavity geometry, adhering to a conformational selection mechanism. This work elucidates the synergistic interplay between conformational dynamics and localized structural adaptations governing anion recognition. The findings advance the rational design of tunable, high-affinity anion receptors and deepen the understanding of conformational regulation in supramolecular systems.