Prevalence and pattern of transfusion transmitted infections among blood donors visiting a tertiary care hospital, Pakistan.

OBJECTIVES: This study aimed to assess the prevalence and patterns of HIV, HBV, HCV, and syphilis among blood donors in Pakistan. METHODS: Data records of all registered blood donors (n = 63,847) during August 2020-April 2025, at a blood transfusion center in a tertiary care hospital were retrospectively analyzed. Prevalence of the seropositive donors for HIV, HCV, HBV and Syphilis was analyzed. RESULTS: The donors ranged in age from 18 years to over 65 years, with the vast majority being male (99.47%). Overall prevalence of TTIs was 2.04% in the donor population. Hepatitis B virus (HBV) was the most prevalent TTI (1.12%; 95% CI: 1.04–1.20), followed by hepatitis C virus (HCV) (0.49%; 95% CI: 0.44–0.55), syphilis (0.36%; 95% CI: 0.32–0.41), and human immunodeficiency virus (HIV) (0.07%; 95% CI: 0.05–0.09) respectively. Replacement donations accounted for 59.34% of the total. The largest proportion of donors (48.7%) were aged 26–35 years. The highest annual donation volume occurred in 2023, with 13,534 donors. The most frequent coinfections were HBV–HCV and HBV–syphilis, with seven cases each. CONCLUSION: Among blood donors, HBV was the most prevalent transfusion-transmitted infection, followed by HCV. CLINICAL TRIAL NUMBER: Not applicable.