Access microbiology · 2024
HIV-DRIVES: HIV drug resistance identification, variant evaluation, and surveillance pipeline.
СтатьяНазвание и аннотация: Перевод в очереди
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Kanyerezi Stephen, Sserwadda Ivan, Ssemaganda Aloysious, Seruyange Julius, Ayitewala Alisen, Oundo Hellen Rosette, Tenywa Wilson, Kagurusi Brian A, Tusabe Godwin, Were Stacy, Ssewanyana Isaac, Nabadda Susan, Namaganda Maria Magdalene, Mboowa Gerald
Аннотация
The global prevalence of resistance to antiviral drugs combined with antiretroviral therapy (cART) emphasizes the need for continuous monitoring to better understand the dynamics of drug-resistant mutations to guide treatment optimization and patient management as well as check the spread of resistant viral strains. We have recently integrated next-generation sequencing (NGS) into routine HIV drug resistance (HIVDR) monitoring, with key challenges in the bioinformatic analysis and interpretation of the complex data generated, while ensuring data security and privacy for patient information. To address these challenges, here we present HIV-DRIVES (HIV Drug Resistance Identification, Variant Evaluation, and Surveillance), an NGS-HIVDR bioinformatics pipeline that has been developed and validated using Illumina short reads, FASTA, and Sanger ab1.seq files.