[Pharmacokinetics of three pairs isomers of Caraway in Beagle dogs based on UPLC-MS/MS].

This study established a rapid, sensitive, and convenient ultra-high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS) method for the simultaneous determination of three pairs of isomers(psoralen and isopsoralen, bavachin and isobavachalcone, bavachromene and isobavachromene) in Beagle dog plasma after oral administration of Caraway extract, and investigated their pharmacokinetic characteristics using a non-compartmental analysis method. Separation was achieved on a Phenomenex ACE Excel 3 phenyl column(4.6 mm × 75 mm, 3 μm) with a mobile phase of 0.2% formic acid in methanol and 0.2% formic acid aqueous solution under gradient elution. The three pairs of isomers and internal standards were completely separated within 15 min, and detection was performed in positive ion multiple reaction monitoring mode. The method was validated in terms of selectivity, linearity, precision, accuracy, recovery, matrix effects, and stability, meeting the requirements for quantitative analysis of biological samples. RESULTS:: showed that, after a single oral dose of Caraway in Beagle dogs, the T_(max) of psoralen, isopsoralen, isobavachalcone, bavachromene, and isobavachromene was 0.50-2.00 h, with a T_(1/2) of 1.55-7.08 h. Isopsoralen and psoralen exhibited relatively higher exposure, with C_(max) of 370.65 and 144.25 ng·mL~(-1), and AUC_(0-∞) of 646.17 and 273.34 ng·h·mL~(-1), respectively. Isobavachalcone, isobavachromene, and bavachromene showed relatively lower exposure, with C_(max) of 8.31, 1.61, and 0.74 ng·mL~(-1), and AUC_(0-∞) of 38.34, 5.48, and 2.24 ng·h·mL~(-1), respectively. Bavachin exposure was the lowest, and its pharmacokinetic parameters could not be determined. This study provides a scientific basis for the clinical application and subsequent new drug research of Caraway.