Fertility and sterility · 2025
Efficacy and clinical outcomes of the Cryopiece set in the cryopreservation of single or very low numbers of human sperm.
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Xue Songguo, Gao Yingzhuo, Wang Rongxiang, Tan Seang Lin, Peng Qiuping, Li Da
Аннотация
OBJECTIVE: To demonstrate the efficacy and clinical outcomes of an advanced cryopreservation method for human single or very low numbers of sperm using the Cryopiece set.
DESIGN: A step-by-step demonstration of this method with a narrated video.
SUBJECTS: This video features patients diagnosed with cryptozoospermia, followed by sperm cryopreservation, thawing, and intracytoplasmic sperm injection (ICSI) using the Cryopiece set.
EXPOSURE: The Cryopiece set includes an innovatively designed dish featuring a specialized groove and a Cryopiece set serving as the sperm cryopreservation carrier. The Cryopiece set can be precisely placed in the groove. A cryoprotectant droplet is placed at the center of the Cryopiece set located in the dish. Sperm are captured in the sample droplets and then transferred to the cryoprotectant droplet using an ICSI needle. The Cryopiece set is then placed in a vial, sealed, and subjected to rapid freezing in the Intelligent Freezing Operation Instrument. For thawing, remove the Cryopiece from the vial and immerse it in 37 °C mineral oil. The cryoprotectant droplet dissolves completely. Prepare the ICSI dish from the Cryopiece set. Transfer the thawed Cryopiece set to its groove, and perform ICSI after standard procedures. The injected oocytes are artificially activated using ionomycin.
MAIN OUTCOME MEASURES: Sperm recovery rate, fertilization rate, cleavage rate, implantation rate, and clinical pregnancy rate.
RESULTS: A total of 1357 sperm from the semen samples of 46 patients with cryptozoospermia were cryopreserved using the Cryopiece set before oocyte retrieval. Experienced operators averaged 2.9 minutes per sperm capture. A total of 179 sperm from 16 couples were thawed, and 167 (93.3%) were successfully recovered. Ninety-four oocytes were injected with thawed sperm. The results demonstrate that Cryopiece-cryopreserved sperm achieves clinical outcomes comparable with those of fresh sperm during the same period, with fertilization rates of 75.5% vs. 79.9% (P=.34), cleavage rates of 93.0% vs. 97.6% (P=.24), implantation rates of 36.0% vs. 46.7% (P=.43), and clinical pregnancy rates of 54.5% vs. 61.1% (P=1.00).
CONCLUSION: The Cryopiece set effectively cryopreserves human single or rare sperm, integrates smoothly into ICSI operations, and achieves satisfactory clinical outcomes.
DESIGN: A step-by-step demonstration of this method with a narrated video.
SUBJECTS: This video features patients diagnosed with cryptozoospermia, followed by sperm cryopreservation, thawing, and intracytoplasmic sperm injection (ICSI) using the Cryopiece set.
EXPOSURE: The Cryopiece set includes an innovatively designed dish featuring a specialized groove and a Cryopiece set serving as the sperm cryopreservation carrier. The Cryopiece set can be precisely placed in the groove. A cryoprotectant droplet is placed at the center of the Cryopiece set located in the dish. Sperm are captured in the sample droplets and then transferred to the cryoprotectant droplet using an ICSI needle. The Cryopiece set is then placed in a vial, sealed, and subjected to rapid freezing in the Intelligent Freezing Operation Instrument. For thawing, remove the Cryopiece from the vial and immerse it in 37 °C mineral oil. The cryoprotectant droplet dissolves completely. Prepare the ICSI dish from the Cryopiece set. Transfer the thawed Cryopiece set to its groove, and perform ICSI after standard procedures. The injected oocytes are artificially activated using ionomycin.
MAIN OUTCOME MEASURES: Sperm recovery rate, fertilization rate, cleavage rate, implantation rate, and clinical pregnancy rate.
RESULTS: A total of 1357 sperm from the semen samples of 46 patients with cryptozoospermia were cryopreserved using the Cryopiece set before oocyte retrieval. Experienced operators averaged 2.9 minutes per sperm capture. A total of 179 sperm from 16 couples were thawed, and 167 (93.3%) were successfully recovered. Ninety-four oocytes were injected with thawed sperm. The results demonstrate that Cryopiece-cryopreserved sperm achieves clinical outcomes comparable with those of fresh sperm during the same period, with fertilization rates of 75.5% vs. 79.9% (P=.34), cleavage rates of 93.0% vs. 97.6% (P=.24), implantation rates of 36.0% vs. 46.7% (P=.43), and clinical pregnancy rates of 54.5% vs. 61.1% (P=1.00).
CONCLUSION: The Cryopiece set effectively cryopreserves human single or rare sperm, integrates smoothly into ICSI operations, and achieves satisfactory clinical outcomes.