Archives of oral biology · 2026
Remodeling of the sagittal suture development in sclerostin knockout male mice associated with cranial flat bone growth.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Chao Fan-Yi, Shimoe Saiji, Mine Yuichi, Taji Tsuyoshi, Nikawa Hiroki, Shukunami Chisa, Kaku Masato
Аннотация
OBJECTIVES: The purpose of this study was to examine the effect of lack of sclerostin gene (Sost) on the growth and development of cranial bone associated with sagittal suture.
DESIGN: 10, 15, 30, and 60-day-old wild-type mice and Sost knockout (SostΔ26/Δ26) mice were served in this study. The calvariae including sagittal sutures were removed and observed microscopically. The activity of osteoclast was examined by tartrate-resistant acid phosphatase (TRAP) staining and the expression of sclerostin and β-catenin were evaluated by immunohistochemical methods.
RESULTS: It was shown that SostΔ26/Δ26 mice resulted in increased bone formation, leading to thickening of the calvaria. Suture stenosis and partial bone obliteration accompanied by higher expressive levels of β-catenin have also been observed in SostΔ26/Δ26 mice, while sagittal suture remains its patency in the late developmental stage.
CONCLUSIONS: These results suggest that SostΔ26/Δ26 mice shows accumulation of β-catenin, inducing a metabolic effect on bone remodeling during the early postnatal period, which may be related to the abnormality of cranial suture in SostΔ26/Δ26 mice.
DESIGN: 10, 15, 30, and 60-day-old wild-type mice and Sost knockout (SostΔ26/Δ26) mice were served in this study. The calvariae including sagittal sutures were removed and observed microscopically. The activity of osteoclast was examined by tartrate-resistant acid phosphatase (TRAP) staining and the expression of sclerostin and β-catenin were evaluated by immunohistochemical methods.
RESULTS: It was shown that SostΔ26/Δ26 mice resulted in increased bone formation, leading to thickening of the calvaria. Suture stenosis and partial bone obliteration accompanied by higher expressive levels of β-catenin have also been observed in SostΔ26/Δ26 mice, while sagittal suture remains its patency in the late developmental stage.
CONCLUSIONS: These results suggest that SostΔ26/Δ26 mice shows accumulation of β-catenin, inducing a metabolic effect on bone remodeling during the early postnatal period, which may be related to the abnormality of cranial suture in SostΔ26/Δ26 mice.