Chemical Profiles of the Ethanol Extract of Coreopsis Tinctoria Nutt. Based on UHPLC-LTQ-Orbitrap-MS/MS and MS3 and its Protective Effect on MGO-Induced Damage to HaCaT Cells.

Coreopsis tinctoria Nutt. (CtN) has a widely used in traditional medicine and food applications in China. As a newly authorized food ingredient in China, CtN provides a safe, natural, and multitarget therapeutic strategy for skin aging intervention, offering unique advantages for the development of 'beauty-from-within' functional foods. However, there is currently a lack of its effects on skin glycation aging. The alcohol extract of Chinese C. tinctoria Nutt (CtNE) was analyzed using UHPLC-LTQ-Orbitrap-MS/MS-(MS2) and MS3 technology. A methylglyoxal (MGO)-induced glycation aging model was established in HaCaT cells, followed by intervention with the CtNE. A total of 28 components were identified from CtNE, which demonstrated the ability to improve MGO-induced cell proliferation inhibition. CtNE increased the number of cells in the S phase, promoted cell migration, reduced reactive oxygen species (ROS) and malondialdehyde (MDA) levels, elevated the antioxidant superoxide dismutase (SOD) level, decreased β-galactosidase activity, and mitigated the nuclear accumulation of K9 trimethylated histone H3 (K9M-H3). Furthermore, CtNE downregulated P21 expression and phosphorylation of P53, while upregulating the expression of P-PI3K,P-AKT,P-mTOR,ZO-1 and Occludin, and inhibiting P-P53 expression. The constituents of CtNE exhibit the potential to ameliorate skin glycation-induced aging.