Fertility and sterility · 2004
Effect of angiotensin-converting enzyme-inhibiting therapy on the expression of vascular endothelial growth factor in hyperstimulated rat ovary.
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Ozcakir Hasan Tayfun, Giray Seren Gulsen, Ozbilgin Mahmut Kemal, Inceboz Umit Sungurtekin, Caglar Husnu
Аннотация
OBJECTIVE: To determine the effect of angiotensin-converting enzyme-inhibiting therapy on the expression of vascular endothelial growth factor (VEGF) in the hyperstimulated rat ovary.
DESIGN: Experimental study.
SETTING: University animal research laboratory.
ANIMAL(S): Thirty Wistar albino adult female rats were studied; 20 rats were stimulated with gonadotropins (groups 1 and 2), and 10 were controls (group 3). Ten of the stimulated rats received additional treatment with enalapril (group 2).
INTERVENTION(S): At the end of the treatment period, rat ovaries were subjected to immunohistochemical staining with anti-VEGF antibodies.
MAIN OUTCOME MEASURE(S): VEGF staining intensity was graded semiquantitatively, and the H-score was calculated by light microscopic examination of the groups.
RESULT(S): VEGF expression was found to be significantly higher in the endothelium and stroma in groups 1 and 2 compared with group 3. Although VEGF immunoreactivity was lower in the stimulation regimen plus enalapril group compared with the stimulation regimen-only group, the difference was insignificant.
CONCLUSION(S): Enalapril does not seem to have a significant effect on VEGF expression in the hyperstimulated rat ovary. Because angiotensin II exerts its multiple actions via specific receptors, there may be other factors, such as a receptor blockade, that contribute to the VEGF expression.
DESIGN: Experimental study.
SETTING: University animal research laboratory.
ANIMAL(S): Thirty Wistar albino adult female rats were studied; 20 rats were stimulated with gonadotropins (groups 1 and 2), and 10 were controls (group 3). Ten of the stimulated rats received additional treatment with enalapril (group 2).
INTERVENTION(S): At the end of the treatment period, rat ovaries were subjected to immunohistochemical staining with anti-VEGF antibodies.
MAIN OUTCOME MEASURE(S): VEGF staining intensity was graded semiquantitatively, and the H-score was calculated by light microscopic examination of the groups.
RESULT(S): VEGF expression was found to be significantly higher in the endothelium and stroma in groups 1 and 2 compared with group 3. Although VEGF immunoreactivity was lower in the stimulation regimen plus enalapril group compared with the stimulation regimen-only group, the difference was insignificant.
CONCLUSION(S): Enalapril does not seem to have a significant effect on VEGF expression in the hyperstimulated rat ovary. Because angiotensin II exerts its multiple actions via specific receptors, there may be other factors, such as a receptor blockade, that contribute to the VEGF expression.