Simultaneous determination of six antibiotics belonging to four different classes in chicken meat BY HPLC/DAD and verification BY LC-MS/MS.

The hypothesis of this study was to develop an extraction method allowing side-by-side extraction of six antibiotics belonging to four different classes and an HPLC/DAD method for the determination of their residues in chicken breast meat. The validation data showed that this hypothesis was achieved. The results were then confirmed by LC-MS/MS method. Sample preparation was based on the classical solid-liquid extraction with methanolic citric acid. The average recoveries were satisfactory and ranged from 75.68 to 101.3%. The linearity of the developed HPLC/DAD method was very high in the concentration range studied (R2 > 0.9969). The accuracy and precision of the analytical method were between -10.8 and 12.0% relative error and 0.82 to 10.1% relative standard deviation, respectively. The LODs for five antibiotics ranged from 0.6 to 2.7 µg kg-1 and LOQs ranged from 2.0 to 8.9 µg kg-1. For penicillin G, the LOD was 0.16 and LOQ was 0.52 mg kg-1.