Cheilanthenetetraol, a sesterterpenoid isolated from Aleuritopteris bicolor with anti-hepatocellular carcinoma and anti-inflammatory activity.

The 1H-NMR, LC-MS, and HPLC guided isolation of n-hexane and ethyl acetate fractions led to the purification of one new sesterterpene cheilanthenetetraol (1) together with thirteen known compounds (2-14) for the first time from Aleuritopteris bicolor Roxb. The structure of the isolated compounds were established by extensive analysis of spectroscopic data, 1D and 2D NMR, HR-ESI-MS, and IR. The isolated compounds (2, 3, 6, and 9-14), as well as the n-hexane, ethyl acetate fractions, and aqueous methanol extract, were evaluated for cytotoxic activity against the HepG2 cancer cell line. All the tested compounds exhibited significant cytotoxic activity with an IC50 ranging from 10.08 µM to 14.04 µM. The extracts exhibited moderate activity with an IC50 value of 315.68, 391.55, and 274.89 µg/mL, respectively. The anti-inflammatory activity of compounds 6, 9, and 10 were assessed, and the results demonstrated that these compounds exhibited significant activity at 10 and 30 µM concentrations in LPS-stimulated macrophages.