Enzyme-Assisted Ultrasonic Extraction of a Polysaccharide-Rich Extract from Abelmoschus manihot (L.) Root and Preliminary Evaluation of Hair Care-Related Properties.

Abelmoschus manihot (L.) flowers are widely used in traditional Chinese medicine, whereas the roots are often discarded as agricultural waste. The compact cellular and tissue architecture of the roots hinders the release of intracellular metabolites, thus impeding their reutilization. Here, enzyme-assisted ultrasonic extraction was developed to obtain a polysaccharide-rich crude extract from A. manihot roots. Orthogonal optimization identified pH 4.5, a temperature of 60 °C and a cellulase dosage of 6% (w/w) as the optimal extraction conditions, giving an extraction yield of 21.59%. The phenol-sulfuric acid assay indicated a total carbohydrate content of 75.09% (glucose equivalents). Structural analysis by Fourier transform infrared (FT-IR) confirmed the presence of characteristic polysaccharide functional groups, while monosaccharide composition analysis via high-performance liquid chromatography (HPLC) revealed the major components of glucose, glucuronic acid, rhamnose, galacturonic acid, etc. Inspired by the traditional use of A. manihot extract in papermaking, its preliminary hair care-related properties were further evaluated. At a concentration of 30 mg/mL, the extract solution inhibited Malassezia furfur growth by 84%, reduced wet and dry combing work by 16.93% and 30.86%, and increased tensile strength and tensile fracture energy by 13.04% and 14.84%, respectively. Scanning electron microscope (SEM) images suggested smoother hair fiber surfaces after treatment. These results highlight the extract's great potential for hair care cosmetic products.