Neurological research · 2007
Differential expression of capillary VEGF isoforms following traumatic brain injury.
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Dore-Duffy Paula, Wang Xueqain, Mehedi Afroza, Kreipke Christian W, Rafols José A
Аннотация
OBJECTIVES: While it is known that angiogenesis occurs after trauma, we sought to characterize the expression of vascular endothelial growth factor (VEGF) subtypes, vascular endothelial growth factor receptor 2 (VEGFR2) and angiopoietin within capillaries of animals subjected to traumatic brain injury (TBI). Further, we sought to characterize pericyte cell death in isolated capillaries.
METHODS: We used Marmarou's acceleration impact model to induce head trauma and measured VEGF, VEGFR2 and angiopoietin levels in isolated capillaries. TUNEL was used to determine pericyte cell death.
RESULTS: The VEGF response was restricted to the VEGF120 isoform. No increase in transcripts for VEGF164 and VEGF188 was observed. VEGFR2 was marginally increased and angiopoietin was increased. A subset of pericytes were TUNEL-positive.
DISCUSSION: These results show a distinct expression pattern of angiogenic factors following injury and suggest that pericyte involvement in adaptive angiogenesis may be altered following TBI.
METHODS: We used Marmarou's acceleration impact model to induce head trauma and measured VEGF, VEGFR2 and angiopoietin levels in isolated capillaries. TUNEL was used to determine pericyte cell death.
RESULTS: The VEGF response was restricted to the VEGF120 isoform. No increase in transcripts for VEGF164 and VEGF188 was observed. VEGFR2 was marginally increased and angiopoietin was increased. A subset of pericytes were TUNEL-positive.
DISCUSSION: These results show a distinct expression pattern of angiogenic factors following injury and suggest that pericyte involvement in adaptive angiogenesis may be altered following TBI.
Предметные рубрики MeSH
ActinsAngiopoietin-1AnimalsApoptosisBehavior, AnimalBrain InjuriesCapillariesCentral Nervous SystemDisease Models, AnimalGene Expression RegulationHypoxiaIn Situ Nick-End LabelingMalePericytesProtein IsoformsRatsRats, Sprague-DawleyTime FactorsVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2