Changes in morphology of human skin fibroblasts induced by local anaesthetics: role of actomyosin contraction.

Local anaesthetics block action potentials in the membranes of excitable cells but their effects on non-excitable cells are less well known. Some local anaesthetics are applied directly onto the skin, and for this reason the effect of procaine (p-aminobenzoic acid diethylamino-etyl ester hydrochloride) and tetracaine (4-[butylamino]benzoic acid 2-[dimethylamino]ethyl ester) upon the morphology and cytoskeleton organisation of human skin fibroblasts was investigated. The time lapse video recording of fibroblasts cultured in serum-enriched medium revealed that the cells rapidly change shape after the addition of the anaesthetic. These effects were fully reversible. The microscopic observations were confirmed by quantitative analysis of projected cell area and cell shape parameters. Local anaesthetics significantly changed the actin cytoskeleton organisation, inducing total disappearance of stress fibres. Serum-starvation or myosin light chain kinase inhibitors, KT 5926 inhibitor (8R*,9S*,11S*)-(-)-9-hydroxy-9-methoxycarbonyl-8-methyl-14-n-propoxy-2,3 ,9, 10-tetrahydro-8,11-epoxy,1H,8H,11H-2,7b,11a-triazadibenzo[a,g]c ycloocta[cde] trinden-1-one or wortmannin, which induce the 'relaxed' morphology of the cells, prevent both the anaesthetic-induced changes in cell shape and the disassembly of stress fibres. Together, the observations suggest that local anaesthetics affect the actomyosin system, inducing contraction.