Multiscale Analysis Reveals Hippocampal Subfield Vulnerabilities to Chronic Cortisol Overexposure: Evidence From Cushing's Disease.

BACKGROUND

Chronic cortisol overexposure plays a significant role in the development of neuropathological changes associated with neuropsychiatric and neurodegenerative disorders. The hippocampus, the primary target of cortisol, may exhibit characteristic regional responses due to its internal heterogeneity. In this study, we explored structural and functional alterations of hippocampal (HP) subfields in Cushing's disease (CD), an endogenous model of chronic cortisol overexposure.

METHODS

Utilizing structural and resting-state functional magnetic resonance imaging data from 169 participants (86 patients with CD and 83 healthy control participants [HCs]) recruited from a single center, we investigated specific structural changes in HP subfields and explored the functional connectivity alterations driven by these structural abnormalities. We also analyzed potential associative mechanisms between these changes and biological attributes, neuropsychiatric representations, cognitive function, and gene expression profiles.

RESULTS

Compared with HCs, patients with CD exhibited significant bilateral volume reductions in multiple HP subfields. Notably, volumetric decreases in the left HP body and tail subfields were significantly correlated with cortisol levels, Montreal Cognitive Assessment scores, and quality of life measures. Disrupted connectivity between the structurally abnormal HP subfields and the ventromedial prefrontal cortex may impair reward-based decision making and emotional regulation, with this dysconnectivity being linked to structural changes in right HP subfields. Another region that exhibited dysconnectivity was located in the left pallidum and putamen. Gene expression patterns associated with synaptic components may underlie these macrostructural alterations.

CONCLUSIONS

Our findings elucidate the subfield-specific effects of chronic cortisol overexposure on the hippocampus, enhancing understanding of shared neuropathological traits linked to cortisol dysregulation in neuropsychiatric and neurodegenerative disorders.