Fertility and sterility · 2011
Prediction of human blastocyst development from morulas with delayed and/or incomplete compaction.
Оценочное исследованиеСтатьяНазвание и аннотация: Перевод в очереди
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Ivec Martin, Kovacic Borut, Vlaisavljevic Veljko
Аннотация
OBJECTIVE: To determine the influence of delayed compaction and fragmentation on the developmental capacity of morulas.
DESIGN: Prospective study.
SETTING: University IVF center.
PATIENT(S): Intracytoplasmic sperm injection (ICSI) cycles with compact embryos on day 4 or day 5.
INTERVENTION(S): The embryos were divided into day 4 (n = 329) and day 5 (n = 256) morulas and graded I, II, or III, according to the percentage of fragmentation (<5%, 5%-20%, or >20%). The embryos were measured using Cronus3 software.
MAIN OUTCOME MEASUREMENT(S): Blastocyst development rate, blastocoel expansion rate, and optimal blastocyst rate. In an optimal blastocyst: surface area, trophectoderm cell number, inner cell mass (ICM) surface area, ICM volume and ICM shape.
RESULT(S): Day 4 morulas in classes I-III developed into optimal blastocysts in 57.4%, 50%, and 35.6% of the total, respectively, and day 5 morulas in classes I-III in 43.3%, 29.1%, and 13.6% of the total, respectively. A negative association was identified between the amount of morula fragmentation, the blastocyst ICM size, and the number of trophectoderm cells. A delay of 1 day in compaction was associated with a reduced ICM volume.
CONCLUSION(S): The measurement of compaction timing and cytoplasmic loss in morulas assists in predicting their ability to develop into optimal blastocysts.
DESIGN: Prospective study.
SETTING: University IVF center.
PATIENT(S): Intracytoplasmic sperm injection (ICSI) cycles with compact embryos on day 4 or day 5.
INTERVENTION(S): The embryos were divided into day 4 (n = 329) and day 5 (n = 256) morulas and graded I, II, or III, according to the percentage of fragmentation (<5%, 5%-20%, or >20%). The embryos were measured using Cronus3 software.
MAIN OUTCOME MEASUREMENT(S): Blastocyst development rate, blastocoel expansion rate, and optimal blastocyst rate. In an optimal blastocyst: surface area, trophectoderm cell number, inner cell mass (ICM) surface area, ICM volume and ICM shape.
RESULT(S): Day 4 morulas in classes I-III developed into optimal blastocysts in 57.4%, 50%, and 35.6% of the total, respectively, and day 5 morulas in classes I-III in 43.3%, 29.1%, and 13.6% of the total, respectively. A negative association was identified between the amount of morula fragmentation, the blastocyst ICM size, and the number of trophectoderm cells. A delay of 1 day in compaction was associated with a reduced ICM volume.
CONCLUSION(S): The measurement of compaction timing and cytoplasmic loss in morulas assists in predicting their ability to develop into optimal blastocysts.