Sexually transmitted diseases · 2025
Persistence of Genetically Related Neisseria gonorrhoeae Strains Causing Disseminated Gonococcal Infection Cases in Southwestern Michigan During 2019 to 2023.
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Joseph Sandeep J, Quilter Laura A S, Raphael Brian H, Cartee John C, Hebrank Katherine, Gernert Kim M, Schmerer Matthew W, Ridpath Alison D, Burgess Elizabeth, Dybas Leslie, Blankenship Heather M, Jones Kelly, Kent James B, Dietrich Stephen, Bernstein Kyle T, Kersh Ellen N, Neisseria gonorrhoeae Working Group
Аннотация
BACKGROUND: Disseminated gonococcal infection (DGI) is a rare complication of Neisseria gonorrhoeae (Ng) infection, historically affecting 0.5% to 3% of individuals. In 2019, a DGI genomic cluster associated with the ST-7822 strain was identified in southwest Michigan. This study retrospectively assessed the persistence of this strain.
METHODS: Ninety Ng isolates from disseminated sites of infection from Michigan (2019-2023) were sequenced; antimicrobial susceptibility testing was performed. Phylogenetic analysis was performed to assess genetic relatedness. Data from 370 ST-7822 urogenital and extragenital gonococcal infection (UGI) isolates were included in the phylogenetic analysis. The porB1A allele, a marker associated with DGI, and antimicrobial resistance markers were also analyzed.
RESULTS: Phylogenetic analysis identified 7 DGI clades, with clade 7 (n = 26) containing the 2019 cluster (n = 11). Clade 7 isolates (mean SNP difference, 32) formed 2 subclusters from a single ancestor between 2019 and 2023 and clustered with local UGI isolates. All clade 7 DGI isolates and 82.7% of ST-7822 UGI isolates carried the porB1A allele. Among DGI isolates with antimicrobial susceptibility testing data (n = 88), all were susceptible to ceftriaxone; no mosaic penA alleles were identified.
CONCLUSIONS: The persistence of clade 7 associated with the 2019 Michigan DGI cluster suggests ongoing regional transmission of the ST-7822 strain type that has a high proportion with porB1A allele, which has been associated with invasive infection. The genetic similarity between DGI and UGI isolates suggests that DGI strains are not genetically distinct but derived from circulating strains causing mucosal infections. Continued genomic surveillance is essential to understand virulence factors contributing to disseminated infections.
METHODS: Ninety Ng isolates from disseminated sites of infection from Michigan (2019-2023) were sequenced; antimicrobial susceptibility testing was performed. Phylogenetic analysis was performed to assess genetic relatedness. Data from 370 ST-7822 urogenital and extragenital gonococcal infection (UGI) isolates were included in the phylogenetic analysis. The porB1A allele, a marker associated with DGI, and antimicrobial resistance markers were also analyzed.
RESULTS: Phylogenetic analysis identified 7 DGI clades, with clade 7 (n = 26) containing the 2019 cluster (n = 11). Clade 7 isolates (mean SNP difference, 32) formed 2 subclusters from a single ancestor between 2019 and 2023 and clustered with local UGI isolates. All clade 7 DGI isolates and 82.7% of ST-7822 UGI isolates carried the porB1A allele. Among DGI isolates with antimicrobial susceptibility testing data (n = 88), all were susceptible to ceftriaxone; no mosaic penA alleles were identified.
CONCLUSIONS: The persistence of clade 7 associated with the 2019 Michigan DGI cluster suggests ongoing regional transmission of the ST-7822 strain type that has a high proportion with porB1A allele, which has been associated with invasive infection. The genetic similarity between DGI and UGI isolates suggests that DGI strains are not genetically distinct but derived from circulating strains causing mucosal infections. Continued genomic surveillance is essential to understand virulence factors contributing to disseminated infections.