Sexually transmitted infections · 2025
Exploring alternative therapeutic options for multidrug-resistant N. Gonorrhoeae: the potential of 7,8-dihydroxyflavone.
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Qi Yuqiu, Xie Qinghui, Lin Jingxia, Zhang Ran, Tang Yingxian, Wang Zhuo, Lin Xiaomian
Аннотация
OBJECTIVES: Neisseria gonorrhoeae (NG), a bacterium characterised by multidrug resistance, is traditionally treated with ceftriaxone, either as a monotherapy or in conjunction with azithromycin. However, the emergence of strains resistant to these antibiotics presents a considerable challenge to current therapeutic approaches, which necessitates the exploration of alternative treatment strategies.
METHODS: Agar dilution and broth microdilution to determine the minimum inhibitory concentrations (MICs) of antibiotics and 7,8-dihydroxyflavone. The development of 7,8-dihydroxyflavone drug resistance was detected under the treatment of sublethal concentration for 30 passages. Genotypic analysis was conducted to examine penA, 23S rRNA alleles and multilocus sequence typing.
RESULTS: 55 clinical NG isolates emphasised the high-level rates of the current antimicrobial resistance, with a notable prevalence of the penA-60.001 FC428 clone and high-level azithromycin-resistant clones characterised by the 23S rRNA-1 (A2059G) and 23S rRNA-2 (C2611T) alleles. Among the strains that are representative of clinical circulation, 7,8-dihydroxyflavone demonstrated inhibitory activity, with MIC50 and MIC90 values of 10 mg/L and 20 mg/L, respectively, which are unlikely to induce drug resistance in NG.
CONCLUSIONS: 7,8-dihydroxyflavone may represent a promising antimicrobial agent in the context of escalating antibiotic resistance.
METHODS: Agar dilution and broth microdilution to determine the minimum inhibitory concentrations (MICs) of antibiotics and 7,8-dihydroxyflavone. The development of 7,8-dihydroxyflavone drug resistance was detected under the treatment of sublethal concentration for 30 passages. Genotypic analysis was conducted to examine penA, 23S rRNA alleles and multilocus sequence typing.
RESULTS: 55 clinical NG isolates emphasised the high-level rates of the current antimicrobial resistance, with a notable prevalence of the penA-60.001 FC428 clone and high-level azithromycin-resistant clones characterised by the 23S rRNA-1 (A2059G) and 23S rRNA-2 (C2611T) alleles. Among the strains that are representative of clinical circulation, 7,8-dihydroxyflavone demonstrated inhibitory activity, with MIC50 and MIC90 values of 10 mg/L and 20 mg/L, respectively, which are unlikely to induce drug resistance in NG.
CONCLUSIONS: 7,8-dihydroxyflavone may represent a promising antimicrobial agent in the context of escalating antibiotic resistance.