Biomeditsinskaia khimiia · 2025
The SPR analysis of the interaction of inactivated poliovirus vaccine attenuated strains with antibodies.
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Gnedenko O V, Ivin Yu Yu, Piniaeva A N, Zyrina A N, Levin I V, Borisenko N S, Zhdanov D D, Ivanov A S, Lisitsa A V, Ishmukhametov A A, Archakov A I
Аннотация
The interaction of inactivated poliovirus vaccine strains with oriented antibodies immobilized to protein A via Fc fragments has been investigated. Using an SPR biosensor, the kinetic and equilibrium parameters of the interaction of vaccine attenuated polioviruses of the Sabin strains type 1 and type 2, inactivated by various methods were determined. The strongest interaction was observed between polyclonal antibodies to Sabin strain type 2 poliovirus and Sabin strain type 2 poliovirus inactivated with β-propiolactone, KD = 1.04⋅10-11 M, as well as the interaction of monoclonal antibodies to Sabin strain type 1 poliovirus and Sabin strain type 1 poliovirus inactivated with formaldehyde, KD = 1.39⋅10-11 M. The high-affinity interaction of inactivated vaccine polioviruses of the Sabin strains type 1 and type 2 with immobilized antibodies indicates that the D-antigen retained its structure after virus inactivation with β-propiolactone or formaldehyde.