A photo-triggered dual-gas donor of nitric oxide and hydrogen sulfide with fluorescence for real-time monitoring of its release.

Multi-gaseous signal molecules play a significant role in regulating various physiological and pathological processes. Therefore, studying the synergistic effects of multi-gas donors on biological systems is essential. However, different types of gas donors vary significantly in terms of gas release, including location, dose, and flux. These variations can have a dramatic impact on the biological effects of the gases and require further analysis for confirmation. Thus, a controllable dual-gas donor that enables self-reporting would be valuable for studying the biological effects of two species of gaseous signal molecules. Herein, we present a novel photo-triggered donor, NHD545, which releases nitric oxide and hydrogen sulfide simultaneously. Furthermore, the dual-gas release from this donor is accompanied by turn-on fluorescence, which could be utilized to monitor the NO and H2S release by microscopy. With NHD545, it is convenient to investigate the synergistic effect of dual-gas bioavailability in vitro.