Chinese journal of cancer research = Chung-kuo yen cheng yen chiu · 2025
Composite score of PD-1 + CD8 + tumor-infiltrating lymphocytes and CD57 + CD8 + tumor ascites lymphocytes is associated with prognosis and tumor immune microenvironment of patients with advanced high-grade serous ovarian cancer.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
He Tianhui, Zhang Jie, Zeng Lin, Yin Zhongnan, Yu Bo, Zhang Xi, Yang Xiaoxue, Shang Chunliang, Xue Lixiang, Guo Hongyan
Аннотация
OBJECTIVE: The expression of programmed death 1 (PD-1) on CD8+ T cells is associated with their activation and exhaustion, while CD57 serves as a senescence marker. The impact of PD-1+ and CD57+CD8+ T cells on the prognosis of patients with advanced high-grade serous ovarian cancer (HGSOC) remain unclear.
METHODS: We assessed the percentages of PD-1+ and CD57+CD8+ T cells in tumor-infiltrating lymphocytes (TILs, n=85) and tumor ascites lymphocytes (TALs, n=87) using flow cytometry. The optimal cutoffs for these markers in TILs and TALs were determined through the log-rank maximization method. Gene expression analysis elucidated the tumor immune microenvironment (TIME, n=36).
RESULTS: Patients with higher PD-1+CD8+ TILs (>87.8%) exhibited longer platinum-free interval (PFI) and overall survival (OS). In contrast, those with elevated CD57+CD8+ TALs (>28.69%) were more likely to experience chemotherapy and had lower complete remission rates, shorter PFI and OS. PD-1+CD8+ TILs are primarily displayed an effector memory state with strong proliferative and secretory capabilities. Approximately 50% of CD57+CD8+ TALs were terminally differentiated, exhibiting significantly impaired proliferation. Based on the proportions of PD-1+CD8+ TILs and CD57+CD8+ TALs, patients were categorized into good, median and poor prognosis groups, with median PFI of 47.78, 27.29 and 11.96 months, respectively (P<0.0001). Median OS for these groups was not reach, 49.23 and 30.92 months, respectively (P<0.0001). Patients with poor prognosis exhibit significantly reduced CD8+ T cell proportion and increased M2 macrophage in the TIME, alongside downregulation of multiple T cell activation-related pathways.
CONCLUSIONS: Lower levels of PD-1+CD8+ TILs and higher CD57+CD8+ TALs, assessed prior to treatment, correlated with poor prognosis and suppressive TIME in advanced HGSOC.
METHODS: We assessed the percentages of PD-1+ and CD57+CD8+ T cells in tumor-infiltrating lymphocytes (TILs, n=85) and tumor ascites lymphocytes (TALs, n=87) using flow cytometry. The optimal cutoffs for these markers in TILs and TALs were determined through the log-rank maximization method. Gene expression analysis elucidated the tumor immune microenvironment (TIME, n=36).
RESULTS: Patients with higher PD-1+CD8+ TILs (>87.8%) exhibited longer platinum-free interval (PFI) and overall survival (OS). In contrast, those with elevated CD57+CD8+ TALs (>28.69%) were more likely to experience chemotherapy and had lower complete remission rates, shorter PFI and OS. PD-1+CD8+ TILs are primarily displayed an effector memory state with strong proliferative and secretory capabilities. Approximately 50% of CD57+CD8+ TALs were terminally differentiated, exhibiting significantly impaired proliferation. Based on the proportions of PD-1+CD8+ TILs and CD57+CD8+ TALs, patients were categorized into good, median and poor prognosis groups, with median PFI of 47.78, 27.29 and 11.96 months, respectively (P<0.0001). Median OS for these groups was not reach, 49.23 and 30.92 months, respectively (P<0.0001). Patients with poor prognosis exhibit significantly reduced CD8+ T cell proportion and increased M2 macrophage in the TIME, alongside downregulation of multiple T cell activation-related pathways.
CONCLUSIONS: Lower levels of PD-1+CD8+ TILs and higher CD57+CD8+ TALs, assessed prior to treatment, correlated with poor prognosis and suppressive TIME in advanced HGSOC.