Fertility and sterility · 2007
Relationship between time of reperfusion and E-selectin expression, neutrophil recruitment, and germ cell apoptosis after testicular ischemia in a rat model.
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Sukhotnik Igor, Greenblatt Robert, Voskoboinik Katya, Lurie Michael, Coran Arnold G, Mogilner Jorge G
Аннотация
OBJECTIVE: To examine the relationship between the time of reperfusion and neutrophil recruitment, E-selectin expression, and germ cell apoptosis in the ischemic and contralateral testis after testicular ischemia-reperfusion (IR) injury in a rat.
DESIGN: Laboratory study.
SETTING: Research laboratory in a faculty of medicine at Technion-institute of technology in Israel.
ANIMAL(S): Sixty adult Sprague-Dawley rats weighing 250-280 g.
INTERVENTION(S): Testicular IR.
MAIN OUTCOME MEASURE(S): Testicular germ cell apoptosis was assessed by terminal deoxynucleotide transferase-mediated 2'-deoxyuridine 5'-triphosphate nick-end labeling immunohistochemical assay, using an in situ cell death detection kit. The recruitment of polymorphonuclear (PMN) cells was calculated per 100 venules. Expression of E-selectin was determined by using immunohistochemical analysis.
RESULT(S): E-selectin expression and polymorphonuclear-cell recruitment in the contralateral testis increased significantly after 1 hour of reperfusion and then remained unchanged during the first 24-48 hours, followed by a gradual decrease. Germ cell apoptosis in the contralateral testis increased after 6 hours of reperfusion, achieved statistical significance after 24 hours, and decreased after 72 hours of reperfusion.
CONCLUSION(S): Germ cell apoptosis in the contralateral testis increases most significantly within the first 24-48 hours, followed by a gradual decrease, after IR injury. E-selectin expression and neutrophil recruitment increases within the first 6 hours and apparently may initiate the increase in germ cell apoptosis.
DESIGN: Laboratory study.
SETTING: Research laboratory in a faculty of medicine at Technion-institute of technology in Israel.
ANIMAL(S): Sixty adult Sprague-Dawley rats weighing 250-280 g.
INTERVENTION(S): Testicular IR.
MAIN OUTCOME MEASURE(S): Testicular germ cell apoptosis was assessed by terminal deoxynucleotide transferase-mediated 2'-deoxyuridine 5'-triphosphate nick-end labeling immunohistochemical assay, using an in situ cell death detection kit. The recruitment of polymorphonuclear (PMN) cells was calculated per 100 venules. Expression of E-selectin was determined by using immunohistochemical analysis.
RESULT(S): E-selectin expression and polymorphonuclear-cell recruitment in the contralateral testis increased significantly after 1 hour of reperfusion and then remained unchanged during the first 24-48 hours, followed by a gradual decrease. Germ cell apoptosis in the contralateral testis increased after 6 hours of reperfusion, achieved statistical significance after 24 hours, and decreased after 72 hours of reperfusion.
CONCLUSION(S): Germ cell apoptosis in the contralateral testis increases most significantly within the first 24-48 hours, followed by a gradual decrease, after IR injury. E-selectin expression and neutrophil recruitment increases within the first 6 hours and apparently may initiate the increase in germ cell apoptosis.