Nano-Bio Interface-Guided Nanoparticle Protein Corona Antigen for Immunoassays and Immunoimaging in a Complex Matrix.

Engineered nanoparticles are widely used in biological imaging and drug delivery because of their excellent physical and chemical properties, but almost all the original functions of engineered nanoparticles suffer from a complex matrix. Herein, we proposed a strategy of preparing nanoparticle protein corona antigens (NPCAgs) through exposing a magnetic core silicon shell (Fe3O4@SiO2) fluorescent probe to an antigen protein solution, which could reduce the adsorption of nanoparticles (NPs) with other proteins in serum. In the presence of target anti-BSA IgG, a competitive-type displacement reaction was implemented between NPs@BSA and other proteins by target anti-BSA IgG through the specific antigen-antibody reaction. In addition, secondary structure analysis showed that almost all of the NPCAgs retained their natural conformation, which ensured the function of the NPCAgs, specifically capturing an antibody. Therefore, the NPCAgs showed good performance in immunoassays and immunoimaging, which should shed light on the application in imaging and identification of other nanomaterials.