Molecules (Basel, Switzerland) · 2023
Marine Natural Compound (Neviotin A) Displays Anticancer Efficacy by Triggering Transcriptomic Alterations and Cell Death in MCF-7 Cells.
СтатьяНазвание и аннотация: Перевод в очереди
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Saquib Quaiser, Schwaiger Stefan, Alilou Mostafa, Ahmed Sarfaraz, Siddiqui Maqsood A, Ahmad Javed, Faisal Mohammad, Abdel-Salam Eslam M, Wahab Rizwan, Al-Rehaily Adnan J, Stuppner Hermann, Al-Khedhairy Abdulaziz A
Аннотация
We investigated the anticancer mechanism of a chloroform extract of marine sponge (Haliclona fascigera) (sample C) in human breast adenocarcinoma (MCF-7) cells. Viability analysis using MTT and neutral red uptake (NRU) assays showed that sample C exposure decreased the proliferation of cells. Flow cytometric data exhibited reactive oxygen species (ROS), nitric oxide (NO), dysfunction of mitochondrial potential, and apoptosis in sample C-treated MCF-7 cells. A qPCR array of sample C-treated MCF-7 cells showed crosstalk between different pathways of apoptosis, especially BIRC5, BCL2L2, and TNFRSF1A genes. Immunofluorescence analysis affirmed the localization of p53, bax, bcl2, MAPKPK2, PARP-1, and caspase-3 proteins in exposed cells. Bioassay-guided fractionation of sample C revealed Neviotin A as the most active compound triggering maximum cell death in MCF-7, indicating its pharmacological potency for the development of a drug for the treatment of human breast cancer.