Green fabricated bimetallic zinc ferrite nanoparticles mitigate oxidative stress-induced pathogenesis.

Current study evaluates the beneficial role of bio-functionalized zinc ferrite nanoparticles fabricated from an aqueous extract of Decalepis hamiltonii leaves (DHLE.ZnFe2O4 NPs) on sodium nitrite (NaNO2) and Diclofenac (DFC) induced oxidative stress in RBCs and Sprague Dawley male rat models. DHLE.ZnFe2O4 NPs were characterized using PXRD, FTIR, SEM-EDAX, HR-TEM and VSM. The data suggests that, DHLE.ZnFe2O4 NPs were crystalline, ellipsoidal in shape with an average size of 10.95 nm and super paramagnetic in nature. DHLE.ZnFe2O4 NPs exhibited anti-oxidant properties by scavenging DPPH, H2O2 and reducing ferric to ferrous ions. Furthermore, DHLE.ZnFe2O4 NPs normalized key parameters of oxidative stress such as LPO, PCC, TT and anti-oxidant enzymes (SOD & CAT). Similar to the previous in-vitro results, DHLE.ZnFe2O4 NPs restored all the said stress parameters in homogenates of the liver, kidney, pancreas and heart. In addition, DHLE.ZnFe2O4 NPs repaired Diclofenac induced tissue damage in the liver, kidney, pancreas and heart by regulating all biochemical parameters. Most importantly, DHLE.ZnFe2O4 NPs exhibited anti-inflammatory, anti-diabetic, anti-thrombotic activities and were non-toxic to RBCs. In conclusion, DHLE.ZnFe2O4 NPs through its anti-oxidant potential ameliorate oxidative stress induced pathogenesis such as, inflammation, tissue damage, diabetes and thrombosis.