Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion Based Amplification (mSIBA).

Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are among the most prevalent causes of sexually transmitted infections (STIs) worldwide. Timely and accurate diagnosis plays an important role in deciding appropriate treatment and preventing the spread of the infection. Strand invasion based amplification (SIBA), is an established isothermal nucleic acid amplification method for the rapid and accurate detection of infectious diseases. SIBA was applied for the simultaneous detection of CT and NG in less than 1 h. The multiplex SIBA (mSIBA) method displayed high analytical sensitivity and specificity for the detection of CT and NG. Since the method is performed at low and constant temperature, it can therefore be run on portable instruments. SIBA enables rapid screening for CT and NG within point-of-care or central laboratory settings.