Interaction with CYP20-3 limits OPDA flux into jasmonate biosynthesis in wounded leaves of arabidopsis grown at eCO2.

Upon recognition of a stress, such as wounding, a rapid increase in jasmonates leads to plant resistance against necrotrophic pathogens and chewing insect herbivores. This jasmonate burst is weaker in Arabidopsis thaliana plants grown under future predicted carbon dioxide levels (eCO2) compared to todays' levels. Even though foliar levels of jasmonoyl-isoleucine are lower in wounded arabidopsis at eCO2, levels of their precursor, 12-oxo-phytodienoic acid (OPDA), are not affected by atmospheric CO2 levels. We focused on the role of the OPDA-binding protein CYP20-3 in regulating jasmonate levels in wounded rosettes of arabidopsis grown at eCO2. By comparing phytohormone and transcriptomic responses of wounded wildtype plants and cyp20-3 grown under different CO2 conditions, our results suggest that under eCO2, CYP20-3 binds to OPDA to limit flux into jasmonate biosynthesis. As well, the CYP20-3-OPDA-SAT1-OASTL-B complex activates cysteine production which can lead to glutathione biosynthesis to buffer changes in the cellular redox state, dampening wound-associated oxidative stress that leads to jasmonate biosynthesis.