Sequential detection of environmental dissolved oxygen and ATP via dual-switching strategy of an enzyme-hydrogel electrochemical biosensor.

Combined detection of the environmental dissolved oxygen and ATP is critically important, but is technically difficult. This work develops an electrochemical biosensor for sequential detection, via the potential-environment dual switching feature of an enzyme-hydrogel. The single electrochemical biosensor offers linear detection ranges both for DO (0.8-8.0 mg L-1) and ATP (0.05-4 µM).